cdbeb4d1-3b9a-493a-837e-7316550d79f5 Basili Marco CNR IRBIM marco.basili@irbim.cnr.it 2025-05-12T14:20:59 ISO 19139 1.0 WGS84 A metagenomic–based investigation of the planktonic microbial food web in relation with ice melting occurring in the Ross Sea coastal ecosystem 2022-11-01 https://antarcticdatacenter.cnr.it/geonetwork/srv/api/records/fb76df45-1654-46c0-b827-b4252769fe0f The effects of sea ice melting, and the consequent changes in the trophic conditions in Antarctic ecosystems, have been focused on phytoplankton with cross-food web links from krill to penguins, while the consequences on the planktonic microbial food web (viruses, prokaryotes and unicellular eukaryotes), driving biogeochemical cycles, have been largely ignored. This research investigates how changes related to sea ice melting influence the diversity and functioning of planktonic microbes in the Ross Sea coastal ecosystem of Terranova Bay. The objectives are: i) to investigate the temporal changes of planktonic microbial biodiversity, ii) to investigate the biodiversity/ecosystem functioning relationships iii) to assess the role of viruses on biodiversity and biogeochemical cycles, iv) to identify the drivers that shape microbial biodiversity and functions, and v) to shed light on the interactions within the planktonic food web, and their response to the ice melting. META-ICE-ROSS integrates ad hoc sampling with sophisticated methodologies of high-throughput sequencing of microbial DNA (metagenomics), never documented in previous Italian expeditions at Terranova Bay. The sampling includes the collection of seawater beneath the pack ice at increasing distance from the MZ Station and characterised by different thermohaline conditions, on a weekly basis from early November to mid January. The groundbreaking nature of META-ICE-ROSS will allow to provide unprecedented evidences on the role of microbial interactions in the functioning of the Ross Sea coastal ecosystem, to improve knowledge on biological changes due to global climate changes and, nonetheless, to provide a baseline assessment of the functioning of the microbial food webs and carbon sequestration in the pelagic environment in the Ross Sea region, within the context of the Ross Sea Region Marine Protected Area (RSRMPA) and its monitoring and research plan. This project aims at providing new insights on the influence of changes in the thermohaline and trophic conditions, as the consequence of the sea ice melting processes, on biodiversity and functioning of the planktonic microbial food web in Antarctic coastal systems. This project will investigate the microbial responses during intensive field work through cross-cutting multidisciplinary and innovative approaches. In detail, the main objectives of this project are: 1. to investigate the temporal changes of planktonic microbial biodiversity, from viruses to prokaryotes and unicellular eukaryotes, by using high throughput sequencing technologies; 2. to investigate the relationships between microbial biodiversity and key attributes of ecosystem functioning (biomass production, organic matter cycling, nutrient regeneration) 3. to assess the role of viral impact on microbial biodiversity and biogeochemical cycles 4. to identify the main environmental drivers that shape microbial biodiversity and potential metabolic functions (by metagenomic analyses) 5. to shed light on the complex network of interactions within the planktonic food webs, and their response to the ice melting and consequent changes in the environmental conditions 6. to elaborate a new conceptual model of the response of the pelagic microbial food webs in coastal polar ecosystems under future climate change scenarios. Luna Gian Marco CNR IRBIM gianmarco.luna@cnr.it Basili Marco CNR IRBIM marco.basili@irbim.cnr.it https://antarcticdatacenter.cnr.it/geonetwork/srv/api/records/cdbeb4d1-3b9a-493a-837e-7316550d79f5/attachments/IMG_20230104_101929.jpg Ross Sea Tethys Bay Mario Zucchelli Station SCAR Gazetteer of Antarctica 2021-01-01 geonetwork.thesaurus.external.theme.scar_gazetteer_multi Sea regions GEMET - INSPIRE themes, version 1.0 2008-01-01 geonetwork.thesaurus.external.theme.httpinspireeceuropaeutheme-theme ENVIRONMENTAL IMPACTS COASTAL PROCESSES GCMD - Science Keywords 2015-01-01 geonetwork.thesaurus.external.theme.gcmd-sciencekeywords META-ICE-ROSS PNRA18_00101 PNRA project 2024-03-18 geonetwork.thesaurus.external.theme.PNRAproject CNR - Consiglio nazionale delle ricerche SZN - Stazione Zoologica Anton Dohrn Università Politecnica delle Marche OGS - Istituto Nazionale di Oceanografia e di Geofisica sperimentale Research Organization 2024-03-18 geonetwork.thesaurus.external.theme.Research_Organization GCMD - Providers 2023-07-24 geonetwork.thesaurus.external.theme.thesaurus Initiative and Programs 2023-10-02 geonetwork.thesaurus.local.theme.InitiativeandPrograms   Creative-Commons CC BY-NC-SA 4.0         38 1 164.0129 164.1526 -74.7051 -74.667 2022-11-01 2023-02-05 FORMATO_DISTRIBUZIONE_DATI (ASCII, CSV, NetCDF...) 1 ISP-CNR ISP-CNR https://www.pnra.aq/it/project/334/metagenomic-based-investigation-planktonic-microbial-food-web-relation-ice-melting http://metaiceross.irbim.cnr.it/ META-ICE-ROSS GENERAL INFORMATION ABOUT THE DATA ORIGIN AND HOW IT GETS TRANSFORMED ALONG THE WAY FROM THE INSTRUMENT TO THE DATABASE.