Coordinate reference systems
Type of resources
Topics
INSPIRE themes
Keywords
Contact for the resource
Provided by
Years
Formats
Representation types
Update frequencies
status
Scale
-
Antarctic aquatic eukaryotic biodiversity provides an unlimited and largely unexploited source of bioactive molecules that may be utilized for the benefit of human health. The aim of our proposal is to probe antibiotic and anticancer properties of these molecules isolated from a selected panel of freshwater and marine eukaryote species, that can be sampled in the area of MZS with no impact on local biodiversity and can, in large measure, be cultivated and/or maintained in laboratory for long periods at very reduced costs. The sampling of species will be conducted on Protists (ciliates and dinoflagellates), Rotifera (bdelloids), obtaining sponge primmorphs, collecting fish body surface mucus, sequencing transcriptomes from Protists, Rotifers, Echinoderms, and Tunicates to be scanned for genes expressing antimicrobial peptides and enzymesproducing bioactive metabolites. The targets of the project are: i) to sample Protists, Rotifers and macroeukaryotes from marine and freshwater environments, ii) to culture Protists, Rotifers, andobtain demosponge primmorph cultures, iii) to sequence transcriptomes to be added to transcriptomes already obtained by the proposing groupand to identify putative candidate peptides, iv) to obtain purified biochemical fractions from Protists, Rotifers, primmorph and fish surface mucus,v) to test produced fractions and synthetic peptides for their in vitro anticancer and antibiotic activities. The proposal involves three research unitsof the Universities of La Tuscia, Trieste and Camerino, and one unit of the National Research Council in Naples. These units maintain solid and documented relationships of collaboration with a multi-year experience in Antarctic eukaryotic physiology, biochemistry and molecular biology.The proposal can also rely on the official collaboration with the laboratories of Prof. Ian Hawes and Prof. Chris Battershill from the University ofWaikato (NZ), with experience in Antarctic freshwater biology and bioactive molecules.
-
Permafrost hosts a potentially large pool of microorganisms, which is supposed to be the only life forms known to have retained viability over geological time. Thawing of the permafrost renews their physiological activity and exposes ancient life to modern ecosystems (Gilichinsky et al, 2008). The adaptation mechanisms of microorganisms, at species or population level, make them susceptible to extreme environmental conditions. The survival of microorganisms in permafrost raises the question of what constitutes the limit for microbial life (Steven et al., 2006; Wagner 2008).
-
The ANT-Biofilm research project (PNRA16_00105) concerned the study of microbial colonization processes in coastal environments of Terra Nova Bay (Ross Sea), through the analysis of the microbial biofilm (bacteria, microalgae) and macrobenthic settlement on plastic substrates, with the aim of determining their possible variations caused by natural or anthropogenic disturbances (variations in salinity or the presence of contaminants, respectively). Microbial biofilms, which play a key role as a substrate for larval settlement of many species of invertebrates, constitute hot-spots of microbial diversity; and it is also known that the communities a microbial are capable of responding rapidly to changing environmental conditions, acting as potential "sentinels" of natural or anthropic perturbations that recently are threatening the Antarctic biota. During the first year of activity (XXXIII Italian expedition, November 2017) stainless steel structures were fixed on the seabed of Road Bay and Tethys Bay mounting panels of artificial substrates (Polyvinyl Chloride, PVC and PolyEthylene, PE) for colonization, which during the XXXIV expedition (November 2018) were retrieved in order to study the fouling formation processes at different levels of biological complexity (from microbial community including bacteria and microalgae to benthic invertebrates) and to evaluate their evolution in two coastal sites differently exposed to natural or anthropogenic forcings.