Surface ozone data, collected through a UV-absorption analyzer (49i or 49c)

The Concordia Research Station provides a unique location for preparatory activities for future human journey to Mars, to explore microbial diversity at subzero temperatures and monitor the dissemination of human-associated microorganisms within the pristine surrounding environment. The present study was performed in the frame of the BacFinder project (European Space Agency, ESA AO-13-Concordia) with the aim to unravel the environmental- and human-associated microbial diversity in the surrounding of the Concordia Station. This is the first intensive and extensive surface snow sampling performed monthly over a two-year period at three distances (10, 500, and 1000 m) from the Concordia Station, and investigated by a high-throughput sequencing approach. Emphasis was laid on the relation between microbial presence and both seasonality and distance from the Base. Data suggested that if present the anthropogenic impact was below the detection limit of the employed DNA sequencing-based techniques. On the other hand, our study corroborated the use of DNA sequencing for revealing microbial presence in remote and hostile environments, with implications for Planetary Protections and for life-detection in astrobiology relevant targets.

The efficacy and reliability of low-impact technologies were tested to document the occurrence and distribution of Antarctic toothfish in sea-ice covered coastal areas at Terra Nova Bay (Ross Sea). Ancillary information, including main water column parameters (temperature, salinity, density, dissolved oxygen, chlorophyll, and turbidity), was collected at each sampling station.

Trophic interactions underlie coexistence mechanisms between species and affect biodiversity and heavy metal bioaccumulation processes. Sea ice dynamics, which at Terra Nova Bay is characterized by an extraordinary seasonality, drives interspecific interactions. Indeed, the activation of the primary production after sea-ice break up opens alternative trophic pathways for consumers. By means of C and N stable isotope analyses, the present project aims at (i) determining food web structure at Terra Nova Bay at different bathymetries and in opposite sea-ice cover conditions; (ii) evaluating heavy metal bioaccumulation in species along food chains, including fish of commercial interests, both in the presence and absence of photosynthetic primary producers. The hypothesis to be tested is if the activation of primary producers following sea-ice break up significantly modifies the food web structure and stability against species loss, as well as heavy metals concentration along food chains. Data on Antarctic food webs are scarce, and even scarcer is our knowledge on mechanisms of primary and secondary biodiversity loss and biomagnification processes in invertebrates and fish. The present research project will shed light on mechanisms underlying biodiversity maintenance in the Antarctic ecosystem and on risks for human health related to heavy metals accumulation in fish species currently or potentially exploited commercially, also in light of expected changes in the extension of sea ice cover. A valuable reference baseline will be established for future studies at the Italian Antarctic Station and for the Marine Protected Area in the Ross Sea.

We will collect oral samples from the volunteers at the Mario Zucchelli Station at different time points, using safe and not harmfull kits. Samples will be analysed by shotgun metagenomic sequencing, considering only the microbial component.

Isotopic composition (Pb and Sr) in particulate matter (PM10) samples, collected at Concordia with monthly time resolution, since January 2018, obtained by ICP-MS.

The observatory created within the project AMICO records microenvironmental parametrs in rocks and soils of 4 localities in Northern Victorial Land: Battleship Promontory, Richard Nunatak, Trio Nunatak, Mount Bowen. The observatory records in each locality moisture, solar radiation, PAR, wind speed, wind direction, gist speed, and rock, soil and air temperature. Data are collected every two hours and are trasmitted remotely daily. On the website it is possible to navigate and visualize the data.

The fungal communities in rocks and soils of three different localities (Battleship Promontory, Richard Nunatak, Trio Nunatak), where stations are collecting climatic data, have been compared with a DNA metabarcoding approach. The results showed a strong differentiation in the communities diversity and composition between the two substrates, with an higher diversity in soils compared to rocks.

The bacterial communities in rocks and soils of three different localities (Battleship Promontory, Richard Nunatak, Trio Nunatak), where stations are collecting climatic data, have been compared with a DNA metabarcoding approach. In addition samples have been trated with Propidium Mono-Azide (PMA) which binds to DNA of damaged cells and inhibit its replication via PCR. The comparison between samples treated with PMA and samples not treated allows to check the bacterial cells that are intact and potentially alive in these substrates. The results showed a strong differentiation in the communities diversity and composition between the two substrates, with an higher diversity in soils compared to rocks. In addition, the PMA treatment revealed the presence of an high proportion of death cells, whose amplification in traditional metabarcoding approaches obscures the correlation trends in this environment.

This is a compilation of all Concordia station datasets that were/are published in the frame of BSRN. The collection will be updated regularly with recent data. The data are subject to the data release guidelines of BSRN (https://bsrn.awi.de/data/conditions-of-data-release/). Basic and other measurements of radiation at Concordia station (2006-01 et seq) .