Trophic interactions underlie coexistence mechanisms between species, define the functional role of specie within communities, affect biodiversity and bioaccumulation processes of heavy metal. Sea-ice dynamics, which at Terra Nova Bay is characterized by an extraordinary seasonality, drives interspecific interactions and the exchange of materials between ecosystem compartments. Indeed, the activation of the primary production after sea-ice break up opens alternative trophic pathways for consumers. The “next generation SRPs” represent a highly appropriate framework for the present project which follows the results obtained with the previous project ISOBIOTOX (PNRA 2013) and aims at (i) determine topological and functional metrics of sympagic and pelagic food webs at Terra Nova Bay under different conditions of sea-ice coverage along a distance gradient from the nearest open water polynya to areas were the seasonal sea-ice coverage persists longer; (ii) evaluate bioaccumulation and biomagnification of heavy metals in trophic sources at the base of the food web and in target species along food chains, including fishes of commercial interests and top predators, both in the presence and absence of sympagic and pelagic primary producers. High resolution food webs will be reconstructed by means of the simultaneous elemental and isotopic analysis of different elements (C and N) and the bioaccumulation of pollutants. The research program integrates complementary research approaches: (a) Elemental analysis coupled with mass spectrometry for stable isotope analysis (δ13C, δ15N) in animal and vegetal tissues and dead organic matter, (b)analyses of heavy metals accumulation (Chromatography) in the constituent species of the Antarctic food web.

The lidar observatory at Mcmurod, was one of the primary stations of the NDACC network (Network for the Detection of Atmospheric Composition Change) from 2004 to 2010. The lidar observes polar stratospheric clouds at a height between 12 and 26 km typically, from early June until the end of Settember. These PSCs play an important role in the ozone chemistry and promote the depletion of the stratospheric ozone layer. The lidar can detect even very thin PSC layers and can provide important information about their chemical composition. The lidar can be used to study formation and dynamical processes and to provide long term records useful for climate studies.

Vertical profiles along the first three kilometres of atmosphere above the ground (from 300 to 3000 m a.g.l.) of equivalent radar reflectivity factor (Ze), Doppler velocity (W) and Doppler spectral width (Sw) from a 24-GHz vertically pointing Micro Rain Radar MRR-2 by METEK GmbH positioned at Concordia Station (Dome C, Antarctica). The main objective of the FIRCLOUDS project is a complete spectral characterization of cirrus and mixed phase clouds in order to evaluate the radiative models in the FIR regime, where the clouds effect is very strong, and systematic spectral measurements are scarcely available. The micro rain radar (MRR) data allows the determination of the clouds reflectivity and the vertical velocity of ice crystals in the cases of precipitating clouds.

Anthropogenic microparticles (AMs) were found for the first time in specimens of Trematomus bernacchii collected in 1998 in the Ross Sea (Antarctica) and stored in the Antarctic Environmental Specimen Bank. Most of the identified AMs were fibers of natural and synthetic origin. The natural AMs were cellulosic, the synthetic ones were polyester, polypropylene, polypropylene/polyester, and cellulose acetate. The presence of dyes in the natural AMs indicates their anthropogenic origin. Five industrial dyes were identified by Raman spectroscopy with Indigo occurring in most of them (55%). Our research not only adds further data to the ongoing knowledge of pollution levels in the Antarctic ecosystem, it provides an interesting snapshot of the past, highlighting that microplastics and anthropogenic fiber pollution had already entered the Antarctic marine food web at the end of the ‘90 s. These findings therefore establish the foundations for understand the changes in marine litter pollution over time.

The DoCTOr (Dome C Tropospheric Observer) project aims to establish an integrated monitoring system to measure simultaneously, continuously and with a high temporal resolution the water vapor and temperature vertical profiles and the radiative exchanges vs. altitude. The monitoring will enable us to detect both long-term trends and fast-evolving phenomena, the latter useful in the interpretation of the causes of the first. This task is performed mainly through remote sensing techniques, allowing for the study of a highly unperturbed atmospheric sample. The integration of all the deployed instrumentation in a single acquisition system simplifies greatly the data analysis needed to retrieve the final products: The REFIR-PAD spectroradiometer (already operating in dome C since 2011) will provide spectrally-resolved atmospheric downwelling radiances in the mid to far-infrared spectral range, while a laser diode based profiler will characterize the microphysics state of the first 3 km of the atmosphere.A real-time data analysis system based on an atmospheric radiative transfer model will then retrieve from the acquired data the temperature and water vapor profiles and the cloud optical thickness in almost all weather conditions found at Dome C, with a temporal resolution of about 10 minutes.

The research program aims to continue accurate measurements of surface radiative fluxes downwelling and upwelling at Dome-C, within the network Baseline Surface Radiation Network (BSRN https://bsrn.awi.de/), in order to provide broadband measurements of solar radiation short wave (in the three downwelling components direct, diffuse, global and in the global reflected fluxes) and thermal radiation (emitted from the atmosphere and from the surface). These measurements performed throughout the year provide complete information of the radiative regime in the East Antarctic Plateau, as well as its seasonal and interannual variability, and the radiative fluxes are an important input parameter for both the mass balance and regional climate models. In addition, the surface irradiance datasets are required to validate and calibrate at least 10-11 different satellite observations.

The data are related to the ionic composition of atmospheric aerosol continuously collected at Dome C. They refer to PM10 and size-segregated aerosol collected at 2-day and 4-day resolution, respectively. The measured chemical parameters include main and trace inorganic anions and cations and selected organic anions (methanesulphonic acid - MSA).

Antarctic ecosystems have a high number of species, that are closely linked to the presence of sea ice and seasonal cycles. This biodiversity is subject to anthropogenic and natural influences. Zooplankton communities can provide a static snapshot of the health of the ecosystem. Zooplankton samples were collected with a 200 μm mesh net at 3 different sampling points at 80 m depth.

The ANT-Biofilm research project (PNRA16_00105) concerned the study of microbial colonization processes in coastal environments of Terra Nova Bay (Ross Sea), through the analysis of the microbial biofilm (bacteria, microalgae) and macrobenthic settlement on plastic substrates, with the aim of determining their possible variations caused by natural or anthropogenic disturbances (variations in salinity or the presence of contaminants, respectively). Microbial biofilms, which play a key role as a substrate for larval settlement of many species of invertebrates, constitute hot-spots of microbial diversity; and it is also known that the communities a microbial are capable of responding rapidly to changing environmental conditions, acting as potential "sentinels" of natural or anthropic perturbations that recently are threatening the Antarctic biota. During the first year of activity (XXXIII Italian expedition, November 2017) stainless steel structures were fixed on the seabed of Road Bay and Tethys Bay mounting panels of artificial substrates (Polyvinyl Chloride, PVC and PolyEthylene, PE) for colonization, which during the XXXIV expedition (November 2018) were retrieved in order to study the fouling formation processes at different levels of biological complexity (from microbial community including bacteria and microalgae to benthic invertebrates) and to evaluate their evolution in two coastal sites differently exposed to natural or anthropogenic forcings.

We will collect stool samples from the volunteers at the Mario Zucchelli Station at different time points. The samples will be analysed by shotgun metagenomic sequencing, considering only the microbial component.