Antarctic ecosystems have a high number of species, that are closely linked to the presence of sea ice and seasonal cycles. This biodiversity is subject to anthropogenic and natural influences. Zooplankton communities can provide a static snapshot of the health of the ecosystem. Zooplankton samples were collected with a 200 μm mesh net at 3 different sampling points at 80 m depth.

The larval stages can be considered as the link from plankton to benthos. In order to study larval recruitment from zoobenthos, 40 tiles were placed at each of the four sites outlined in the project: Tethys Bay (control), Rod Bay (area subject to anthropogenic impact), Faraglione (control) and Adelie Cove (area subject to natural impact, linked to the presence of a penguin house). Due to adverse environmental factors all the tiles from Faraglione were lost and from Tethys Bay only half were found. The tiles were analysed with the help of a binocular, which enabled better visualisation of the organisms and, consequently, better identification.

The Middle Trophic Level of the Ross Sea is still not well known but it mainly characterized by two species of Krill: Euphausia superba, Euphausia Crystallorophias. These species are a key food source for a wide variety of marine birds and mammals in the Ross Sea. For this reason, it's necessary to improve the knowledge on abundance and spatial distribution of MTL species, and their interconnection with environmental parameters to understand the main drivers of the reproductive success and migrations of these species. In this project, an acoustic survey, a synoptic net sampling and a CTD/XBT samplings were carried out following the standard protocols indicated by CCAMLR. Acoustic monitoring was performed at five frequencies: 38, 70, 120, 200 and 333 kHz. Targeted net samplings were performed to validate the preliminary species allocation of the aggregations observed on the echosounder screen. Biometric measures of krill samples was made on board and samples were taken for further analysis. CTD and XBT data was collected and elaborated to characterize water masses and to study the possible influences of the distribution of the main oceanographic parameters on MTL species.

Antarctic ecosystems have a high number of species, that are closely linked to the presence of sea ice and seasonal cycles. This biodiversity is subject to anthropogenic and natural influences. Macrozoobenthic communities are the 'biological memory' of the ecosystem, structuring themselves as a function of environmental changes over the years. Macrozoobenthos samples were collected using suction dredging and scraping techniques, in soft and hard bottoms. Sampling occurred in 5 different sites.