MIDAS: MIcrobial Diversity within the Vicinity of the Concordia Antarctic Station

The Concordia Research Station provides a unique location for preparatory activities for future human journey to Mars, to explore microbial diversity at subzero temperatures and monitor the dissemination of human-associated microorganisms within the pristine surrounding environment. The present study was performed in the frame of the BacFinder project (European Space Agency, ESA AO-13-Concordia) with the aim to unravel the environmental- and human-associated microbial diversity in the surrounding of the Concordia Station. This is the first intensive and extensive surface snow sampling performed monthly over a two-year period at three distances (10, 500, and 1000 m) from the Concordia Station, and investigated by a high-throughput sequencing approach. Emphasis was laid on the relation between microbial presence and both seasonality and distance from the Base. Data suggested that if present the anthropogenic impact was below the detection limit of the employed DNA sequencing-based techniques. On the other hand, our study corroborated the use of DNA sequencing for revealing microbial presence in remote and hostile environments, with implications for Planetary Protections and for life-detection in astrobiology relevant targets.